Multimodal Imaging Core
The Multimodal Imaging Core provides services to investigators locally, regionally, and nationally, encompassing scales that range from cellular to whole-animal imaging. The goal of the Core is to support the research of investigators in qualitative and quantitative imaging applications and to assist with study design, data interpretation, grant submissions, and to conduct preclinical and IND-enabling studies.
In vivo imaging services provided by Core faculty and trained professionals includes ultrasound imaging, optical imaging, positron emission tomography/computed tomography (PET/CT), and microPET, in addition to radiochemistry and pharmacokinetic analyses of new radiopharmaceuticals.
Dedicated faculty and staff are responsible for imaging services, radiotracer synthesis, administrative support, day-to-day operational management, computer support, preventive and routine maintenance, quality assurance and quality control.
The Core also provides histology (e.g., hematoxylin and eosin staining) and immunohistochemistry services specific to nonhuman primates and services related to whole slide scanning.
- Highly innovative methods for bioluminescence imaging in monkeys for a spectrum of gene therapy and stem cell transplantation studies. These include studies performed for investigators in the NHLBI Center for Fetal Monkey Gene Transfer for Heart, Lung, and Blood Diseases.
- Improved contrast-to-noise imaging performance for cell trafficking studies has been accomplished with the development of new copper (64Cu) and zirconium (89Zr) radioimmunoconjugates for successfully radiolabeling and tracking stem and progenitor cells post-transplantation.
- New PET radiotracers have been developed which are now available in the Core and include 11C-PK11195 and 18F-PBR111 that bind to the translocator protein (TSPO) for inflammation, 18F-FMT for studying dopamine synthesis, and 11C-PIB for amyloid imaging.
Select publications from Multimodal Imaging Core activities
Baker CA, Swainson L, Lin DL, Wong S, Hartigan-O’Connor DJ, Lifson JD, Tarantal AF, and McCune JM. Exposure to SIV in utero results in reduced viral loads and altered responsiveness to postnatal challenge. Sci Transl Med. 2015;7(300):300ra125. PMCID: PMC5100009
Bakkour S, Baker CA, Tarantal AF, Wen L, Busch MP, Lee TH, and McCune JM. Analysis of maternal microchimerism in rhesus monkeys (Macaca mulatta) using real-time quantitative PCR amplification of MHC polymorphisms. Chimerism. 2014;5(1):6-15. PMCID: PMC3988117
Batchelder CA, Duru N, Lee CI, Baker CA, Swainson L, McCune JM, and Tarantal AF. Myeloid-lymphoid ontogeny in the rhesus monkey (Macaca mulatta). Anat Rec (Hoboken). 2014;297(8):1392-406. PMCID: PMC4120262
Batchelder CA, Martinez ML, Duru N, Meyers FJ, and Tarantal AF. Three dimensional culture of human renal cell carcinoma organoids. PLoS One. 2015;10(8):e0136758. PMCID: PMC4552551
Batchelder CA, Martinez ML, and Tarantal AF. Natural scaffolds for renal differentiation of human embryonic stem cells for kidney tissue engineering. PLoS One. 2015;10(12):e0143849. PMCID: PMC4672934
Berg E, Roncali E, and Cherry SR. Optimizing light transport in scintillation crystals for time-of-flight PET: an experimental and optical Monte Carlo simulation study. Biomed Opt Express. 2015;6(6):2220-30. PMCID: PMC4473755
Berg E, Roncali E, Hutchcroft W, Qi J, and Cherry SR. Improving depth, energy and timing estimation in PET detectors with deconvolution and maximum likelihood pulse shape discrimination. IEEE Trans Med Imaging. 2016; 35(11):2436-46. PMCID: PMC5119913
Berg E, Roncali E, Kapusta M, Du J, and Cherry SR. A combined time-of-flight and depth-of-interaction detector for total-body positron emission tomography. Med Phys. 2016;43(2):939-50. PMCID: PMC4733082
Carbonaro Sarracino D, Tarantal AF, Lee CC, Martinez M, Jin X, Wang X, Hardee CL, Geiger S, Kahl CA, and Kohn DB. Effects of vector backbone and pseudotype on lentiviral vector-mediated gene transfer: studies in infant ADA-deficient mice and rhesus monkeys. Mol Ther. 2014;22(10):1803-16. PMCID: PMC4428412
Chaffin CL, Latham KE, Mtango NR, Midic U, and VandeVoort CA. Dietary sugar in healthy female primates perturbs oocyte maturation and in vitropreimplantation embryo development. Endocrinology. 2014; 155(7):2688-95. PMCID: PMC4060180
Conlon T, Mah C, Pacak C, Rucker Henninger M, Erger K, Jorgensen M, Lee C, Tarantal AF, and Byrne BJ. Transfer of therapeutic genes into fetal rhesus monkeys using recombinant adeno-associated type 1 viral vectors. Hum Gene Ther Clin Dev. 2016;27(4):152-9. PMCID: PMC5310237
Corti M, Cleaver B, Clément N, Conlon TJ, Faris KJ, Wang G, Benson J, Tarantal AF, Fuller D, Herzog RW, and Byrne BJ. Evaluation of readministration of a recombinant adeno-associated virus vector expressing acid alpha-glucosidase in Pompe disease: Preclinical to clinical planning. Hum Gene Ther Clin Dev. 2015; 26(3):185-93. PMCID: PMC4606909
Du J, Yang Y, Bai X, Judenhofer MS, Berg E, Di K, Buckley S, Jackson C, and Cherry SR. Characterization of large-area SiPM array for PET applications. IEEE Trans Nucl Sci. 2016;63(1):8-16. PMCID: PMC4281963
Engle JR, Machado CJ, Permenter MR, Vogt JA, Maurer AP, Bulleri AM, and Barnes CA. Network patterns associated with navigation behaviors are altered in aged nonhuman primates. J Neurosci. 2016;36(48):12217-27. PMCID: PMC5148220
Gong K, Cherry SR, and Qi J. On the assessment of spatial resolution of PET systems with iterative image reconstruction. Phys Med Biol. 2016;61(5):N193-202. PMCID: PMC4890626
Hinde K, Muth C, Maninger N, Ragen BJ, Larke RH, Jarcho MR, Mendoza SP, Mason WA, Ferrer E, Cherry SR, Fisher-Phelps ML, and Bales KL. Challenges to the pair bond: Neural and hormonal effects of separation and reunion in a monogamous primate. Front Behav Neurosci. 2016;10:221. PMCID: PMC5107580
Hinderer C, Bell P, Louboutin JP, Zhu Y, Yu H, Lin G, Choa R, Gurda BL, Bagel J, O’Donnell P, Sikora T, Ruane T, Wang P, Tarantal AF, Casal ML, Haskins ME, and Wilson JM. Neonatal systemic AAV induces tolerance to CNS gene therapy in MPS I dogs and nonhuman primates. Mol Ther. 2015;23(8):1298-307. PMCID: PMC4817868
Kwon SI, Ferri A, Gola A, Berg E, Piemonte C, Cherry SR, and Roncali E. Reaching 200-ps timing resolution in a time-of-flight and depth-of-interaction positron emission tomography detector using phosphor-coated crystals and high-density silicon photomultipliers. J Med Imaging (Bellingham). 2016;3(4):043501. PMCID: PMC5120149
Kwon SI, Gola A, Ferri A, Piemonte C, and Cherry SR. Bismuth germanate coupled to near ultraviolet silicon photomultipliers for time-of-flight PET. Phys Med Biol. 2016;61(18):L38-L47. PMCID: PMC5056849
Nicol LE, O’Brien TD, Dumesic DA, Grogan T, Tarantal AF, and Abbott DH. Abnormal infant islet morphology precedes insulin resistance in PCOS-like monkeys. PLoS One. 2014;9(9):e106527. PMCID: PMC4160158
Qu Y, Frazer LC, O’Connell CM, Tarantal AF, Andrews CW Jr, O’Connor SL, Russell AN, Sullivan JE, Poston TB, Vallejo AN, and Darville T. Comparable genital tract infection, pathology, and immunity in rhesus macaques inoculated with wild-type or plasmid-deficient Chlamydia trachomatis serovar D. Infect Immun. 2015; 83(10):4056-67. PMCID: PMC4567646
Tai DS, Hu C, Lee CC, Martinez M, Cantero G, Kim EH, Tarantal AF, and Lipshutz GS. Development of operational immunologic tolerance with neonatal gene transfer in nonhuman primates: preliminary studies. Gene Ther. 2015;22(11):923-30. NIHMSID: NIHMS867303
VandeVoort CA, Gerona RR, Vom Saal FS, Tarantal AF, Hunt PA, Hillenweck A, and Zalko D. Maternal and fetal pharmacokinetics of oral radiolabeled and authentic bisphenol A in the rhesus monkey. PLoS One. 2016; 11(12):e0165410. PMCID: PMC5145146
VandeVoort CA, Grimsrud KN, Midic U, Mtango N, and Latham KE. Transgenerational effects of binge drinking in a primate model: implications for human health. Fertil Steril. 2015;103(2):560-9. PMCID: PMC4314404