Endocrine Core

Endocrine Core 2016-10-14T18:02:55+00:00

The Endocrine Core Laboratory provides an international service for hormone analysis as well as consultation relating to endocrine research for investigators. The Core’s emphasis focuses on the analysis of biological specimens for reproductive and metabolic hormones. In addition, this Core develops and validates unique steroid and protein hormone assays for use in research conducted with nonhuman primates. Services include steroid and protein hormone immunoassays in a variety of species from blood and/or urine samples as well as cell-based bioassays in serum samples.

Capabilities and Resources

  • Sensitive macaque and human chorionic gonadotropin assays for early pregnancy determination
  • Routine reproductive endocrine assays such as serum dehydroepiandrosterone sulfate (DHEAS), estradiol, progesterone and testosterone
  • Urinary metabolites of estrogen, progesterone and follicle stimulating hormone (FSH)
  • Cell-mediated bioassays for total estrogen or androgen receptor ligand loads and luteinizng hormone
  • Metabolic endocrine assays such as adrenocorticotropic hormone (ACTH), cortisol, insulin, prolactin and thyroid hormones
  • Other assays (including analysis of human samples) available upon request – please contact us for additional information
  • Development of new assays as needed
  • Assistance in experimental design and collection/preparation of samples
  • Evaluation of reproductive status

Publications

Kennedy RC, Menn FM, Healy L, Fecteau KA, Hu P, Bae J, Gee NA, Lasley BL, Zhao L, Chen J. Early life triclocarban exposure during lactation affects neonate rat survival. Reprod Sci. 2015 Jan;22(1):75-89. doi: 10.1177/1933719114532844. PMID: 24803507

Yau VM, Lutsky M, Yoshida CK, Lasley B, Kharrazi M, Windham G, Gee N, Croen LA. Prenatal and Neonatal Thyroid Stimulating Hormone Levels and Autism Spectrum Disorders. J Autism Dev Disord Mar 45(3): 719-730, 2015. PMID: 25178989 [PubMed – in process]

Moran FM, Chen J, Gee NA, Lohstroh PN, Lasley BL. Dehydroepiandrosterone sulfate levels reflect endogenous luteinizing hormone production and response to human chorionic gonadotropin challenge in older female macaque (Macaca fascicularis). Menopause 20(3):329-35, 2013. PMCID: PMC3546135, PMID 23435031.

Baxter MG, Roberts MT, Gee NA, Lasley BL, Morrison JH, Rapp PR. Multiple clinically relevant hormone therapy regimens fail to improve cognitive function in aged ovariectomized rhesus monkeys. Neurobiol Aging 34(7):1882-90, 2013. PMCID: PMC3622837, PMID 23369546

Conley AJ, Stanczyk FZ, Morrison JH, Borowicz P, Benirschke K, Gee NA, Lasley BL. Modulation of higher-primate adrenal androgen secretion with estrogen-alone or estrogen-plus-progesterone intervention. Menopause 20(3):322-8, 2013. PMCID: PMC3610787, PMID 23435030

Kundu MC, May MC, Chosich J, Bradford AP, Lasley B, Gee N, Santoro N, Appt SE, Polotsky AJ. Assessment of luteal function in the vervet monkey as a means to develop a model for obesity-related reproductive phenotype. Syst Biol Reprod Med 59(2):74-81, 2013. PMID 23278149

Lasley BL, Crawford SL, McConnell DS. Ovarian adrenal interactions during the menopausal transition. Minerva Ginecol. 2013 Dec;65(6):641-51. PMCID: PMC4417336

Young ME, Ohm DT, Janssen WG, Gee NA, Lasley BL, Morrison JH. Continuously delivered ovarian steroids do not alter dendritic spine density or morphology in macaque dorsolateral prefrontal cortical neurons. Neuroscience 255:219-25, 2013. PMCID: PMC3957098, PMID 24120552

Fan X, Krieg S, Hwang JY, Dhal S, Kuo CJ, Lasley BL, Brenner RM, Nayak NR. Dynamic regulation of Wnt7a expression in the primate endometrium: implications for postmenstrual regeneration and secretory transformation. Endocrinology 153(3):1063-9, 2012. PMCID: PMC3281546, PMID 22294752

Ohm DT, Bloss EB, Janssen WG, Dietz KC, Wadsworth S, Lou W, Gee NA, Lasley BL, Rapp PR, Morrison JH. Clinically relevant hormone treatments fail to induce spinogenesis in prefrontal cortex of aged female rhesus monkeys. J Neurosci 32(34):11700-5, 2012. PMCID: PMC3657730, PMID 22915112

Plopper CG, Joad JP, Miller LA, Schelegle ES, Fanucchi MV, Van Winkle LS, Tyler NK, Avdalovic MV, Evans MJ, Lasley WL, Buckpitt AR, Pinkerton KE, Tarkington BK, Davis S, Nishio SJ, Gershwin LJ, Wu R, Hyde DM. Lung effects of inhaled corticosteroids in a rhesus monkey model of childhood asthma. Clin Exp Allergy. 2012 Jul;42(7):1104-18. PMCID: PMC3913647

Yon L, Faulkner B, Kanchanapangka S, Chaiyabutr N, Meepan S, Lasley B. A safer method for studying hormone metabolism in an Asian elephant (Elephas maximus): accelerator mass spectrometry. Zoo Biol 29(6):760-6, 2010. PMID 20127658

Mohr FC, Lasley B, Bursian S. Fuel oil-induced adrenal hypertrophy in ranch mink (Mustela vison): effects of sex, fuel oil weathering, and response to adrenocorticotropic hormone. J Wildl Dis 46(1):103-10, 2010. PMID 20090023

Mohr FC, Lasley B, Bursian S. Chronic oral exposure to bunker C fuel oil causes adrenal insufficiency in ranch mink (Mustela vison). Arch Environ Contam Toxicol 54(2):337-47, 2008. PMID 17763884

Santoro N, Crawford SL, Lasley WL, Luborsky JL, Matthews KA, McConnell D, Randolph JF Jr, Gold EB, Greendale GA, Korenman SG, Powell L, Sowers MF, Weiss G. Factors related to declining luteal function in women during the menopausal transition. J Clin Endocrinol Metab. 2008 May;93(5):1711-21. PMCID: PMC2386686

Yon L, Chen J, Moran P, Lasley B. An analysis of the androgens of musth in the Asian bull elephant (Elephas maximus). Gen Comp Endocrinol 155(1):109-15, 2008. PMID 17466989

Hao J, Rapp PR, Janssen WG, Lou W, Lasley BL, Hof PR, Morrison JH. Interactive effects of age and estrogen on cognition and pyramidal neurons in monkey prefrontal cortex. Proc Natl Acad Sci U S A 104(27):11465-70, 2007. PMCID: PMC2040921, PMID 17592140

Lohstroh P, Laughlin L, Gee N, Lasley B. Development, validation and application of a chemiluminescent immunoassay for the measurement of circulating chorionic gonadotropin levels in the laboratory macaque. J Med Primatol 36(3):164-9, 2007. PMID 17517091

Yon L, Kanchanapangka S, Chaiyabutr N, Meepan S, Stanczyk FZ, Dahl N, Lasley B. A longitudinal study of LH, gonadal and adrenal steroids in four intact Asian bull elephants (Elephas maximus) and one castrate African bull (Loxodonta africana) during musth and non-musth periods. Gen Comp Endocrinol 151(3):241-5, 2007. PMID 17350012

Yon L, Kanchanapangka S, Chaiyabutr N, Stanczyk F, Meepan S, Lasley B. ACTH stimulation in four Asian bull elephants (Elephas maximus): an investigation of androgen sources in bull elephants. Gen Comp Endocrinol 151(3):246-51, 2007. PMID 17350010

Browne P, Conley AJ, Spraker T, Ream RR, Lasley BL. Sex steroid concentrations and localization of steroidogenic enzyme expression in free-ranging female northern fur seals (Callorhinus ursinus). Gen Comp Endocrinol 147(2):175-83, 2006. PMID 16473352

Santoro N, Torrens J, Crawford S, Allsworth JE, Finkelstein JS, Gold EB, Korenman S, Lasley WL, Luborsky JL, McConnell D, Sowers MF, Weiss G. Correlates of circulating androgens in mid-life women: the study of women’s health across the nation. J Clin Endocrinol Metab. 2005 Aug;90(8):4836-45. PMID: 15840738

Stevens RG, Cohen RD, Terry MB, Lasley BL, Siiteri P, Cohn BA. Alcohol consumption and serum hormone levels during pregnancy. Alcohol 36(1):47-53, 2005. PMID 16257353

Schwartz JA, Aldridge BM, Lasley BL, Snyder PW, Stott JL, Mohr FC. Chronic fuel oil toxicity in American mink (Mustela vison): systemic and hematological effects of ingestion of a low-concentration of bunker C fuel oil. Toxicol Appl Pharmacol 200(2):146-58, 2004. PMID 15476867

Bill Lasley, Ph.D.
ENDOCRINE CORE LEAD

Dr. Lasley has conducted research with rhesus macaques for the past 40 years in reproductive endocrinology and health, and recently has focused on the relation between aging and adrenal steroid production in women’s healthy aging.

His research produced the previously unknown relationship between the ovary and adrenal during the menopausal transition and he has published extensively on this topic. Because of the unique properties of women’s aging, studies such as Dr. Lasley’s are fully addressed only in the primate animal model and cannot be answered in rodents.  He has spent many years developing non-invasive methods for evaluating reproductive function; these methods are now used in many population-based studies and remain the only practical means of documenting normal ovarian function in higher primates, including the human.

 

 

For more information, please contact:

Email: endocore@ucdavis.edu