Research. The FCC supplies flow cytometry instrumentation, experimental protocols, technical support, maintenance, and administration for three instruments that are very intensively used in a variety of research endeavors. Much of the immunology, cell biology, and infectious disease work carried out at the CNPRC employs FCC services. The work of the FCC is guided by a Faculty Advisory Board to ensure that user and faculty needs are met.
Development and consultation. The FCC receives frequent requests for consultation on experiments that push the boundaries of current knowledge or are simply outside the comfort range of most investigators. Often such requests are focused on identification of certain cellular phenotypes in macaques. In these cases the FCC can often provide both guidance and preliminary data; alternatively, we can suggest a plan for developing appropriate panels and analytic approaches.
Training. The FCC trains all core users to ensure safe operation of the instruments and successful collection of data. Crucially, the FCC also provides in-person, on-site support during extended business hours. On-site support is provided so that user difficulties can be addressed quickly and the machines can remain continuously operational.
Service. Certain flow cytometry panels are required by Primate Medicine to provide the best possible animal care. These panels are offered as a service by the CNPRC Clinical Laboratory using FCC instruments.
Dennis Hartigan-O’Connor, M.D., Ph.D.
FLOW CYTOMETRY CORE SCIENTIFIC LEAD
CNPRC CORE SCIENTIST
Infectious Diseases Unit
Reproductive Sciences and Regenerative Medicine
Tracy Rourke is key operator for the CNPRC Flow Cytometry Core. His interests are in application of cutting-edge flow cytometric techniques to clinical and research studies of non-human primate models of disease. Tracy earned his BA degree at UC Davis and devoted his subsequent career to study of non-human primates at the CNPRC. He is co-author on 13 manuscripts relevant to the immunology of SIV-infected non-human primates.