Pathogen Detection Laboratory Core

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Pathogen Detection Laboratory Core 2017-04-13T18:34:02+00:00

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The Pathogen Detection Laboratory offers antibody, antigen, virus, DNA, RNA and other diagnostic marker detection for a number of infectious agents. All methods and reagents are validated, standardized, and controlled by in-laboratory studies and proficiency testing using reference banks of characterized specimens. Quantitation is available by arrangement. Test results are reviewed by clinical laboratory personnel who are fully accredited by the American Society for Clinical Pathology and Laboratory Field Services of the State of California. Custom testing, training, and consultation are available; please inquire. In addition, reagents and controls are available through Resource Services. PDL is a resource for Specific Pathogen-Free (SPF) colonies, field or epidemiological studies, research protocols, and atypical case resolution.

PDL Capabilities and Resources

We offer:

  • Specialty lab testing and expert consultation services to both the public and private sector. This includes local, regional, national and international research colonies; zoological collections; veterinary laboratories; and practices of all sizes.
  • Methods and reagents validated, standardized, and controlled by in-laboratory studies and proficiency testing.
  • Maintenance of reference banks of characterized specimens
  • All test results reviewed by qualified senior personnel prior to reporting
  • Resource for development and maintenance of Specific Pathogen-free (SPF) colonies
  • Resource for field or epidemiological studies
  • Resource for laboratory baseline and monitoring of animals on research studies
  • Resource for unusual or atypical diagnostic cases
  • Staff committed to quality and client-oriented service

 

 

Koen Van Rompay, D.V.M., Ph.D.
PDL SCIENTIFIC LEAD
CNPRC CORE SCIENTIST
Infectious Diseases Unit

Visit Dr. Van Rompay’s page.
Email: kkvanrompay@ucdavis.edu

Jeffery Roberts, D.V.M.
PDL CLINICAL LEAD
CNPRC ASSOCIATE DIRECTOR OF PRIMATE SERVICES

Email: jaroberts@ucdavis.edu

JoAnn Yee, B.S., M.L.S.
TECHNICAL SUPERVISOR

Email: joyee@ucdavis.edu

As lead technician for the Pathogen Detection Laboratory, JoAnn’s scientific interests are in the application of new technologies and diagnostic quality standards to clinical and research studies of infectious disease.

JoAnn earned a BS degree in biochemistry at UC Davis, and is licensed as a clinical lab scientist from the State of California. She also holds a certification as a medical laboratory scientist from the American Society of Pathology. In the early 1980s, JoAnn was the founding technologist for both the AIDS virus diagnostic lab and the forerunner of the PDL. In 2013, she was awarded a prestigious honorary membership in the Association of Primate Veterinarians for her significant contributions to the field.

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Pathogen Detection Laboratory Core is an integral component of research at the CNPRC.

 

Our History…since 1987

Technician

PDL was funded by NCRR under the direction of Dr. Lerche to develop and offer testing for nonhuman primates to develop specific pathogen free colonies and improve overall nonhuman primate colony health.

PDL now functions as both a research and diagnostic laboratory, and provides testing for SPF colonies.

Over the years, the assays developed by PDL have been widely applied and resulted in more accurate and efficient diagnostic tests leading to specific pathogen free and better characterized nonhuman primate colonies. PDL has also taken a leading role in technology transfer to other nonhuman primate institutions by providing training, characterized controls and reagents, and proficiency testing.

We strive at developing new and improved tests as science and technology advance leading to the discovery of new pathogens and more sophisticated technologies.

Publications

Evans TS, Barry PA, Gilard KV, Goldstein T, Deere JD, Fike J, Yee J, Ssebide BJ, Karmacharya D, Cranfield MR, Wolking D, Smith B, Mazet JAK, Johnson CK. Optimization of a novel non-invasive oral sampling technique for zoonotic pathogen surveillance in nonhuman primates. PLOS Published: June 5, 2015 •DOI: 10.1371/journal.pntd.0003813

Yee JL, NPRC Breeding Colony Management Consortium, McChesney MB, Christe KL. Multi-Center Safety and Immunogenicity Trial of an Attenuated Measles Vaccine (MVacTM) for Nonhuman Primates. Comp Med. 2015. In press.

Van Rompay KK, Hunter Z, Jayashankar K, Peabody J, Montefiori D, Labranche CC, Keele BF, Jensen K, Abel K, Chackerian B. A vaccine against CCR5 protects a subset of macaques upon intravaginal challenge with Simian immunodeficiency virus SIVmac251. J Virol 88(4):2011-2024, 2014. PMID 24307581

Jensen K, Pena MG, Wilson RL, Ranganathan UD, Jacobs WR Jr, Fennelly G, Larsen M, Van Rompay KK, Kozlowski PA, Abel K. A neonatal oral Mycobacterium tuberculosis-SIV prime / intramuscular MVA-SIV boost combination vaccine induces both SIV and Mtb-specific immune responses in infant macaques. Trials Vaccinol 2:53-63, 2013. PMID 24454591

Tanaka T, Lerche NW, Farver TB, Ardeshir A, Kass PH. Specific-pathogen-free status is associated with lower infant mortality rate in rhesus macaque (Macaca mulatta) colonies at the California National Primate Research Center. J Med Primatol 42(4):186-91, 2013. PMID 23586439

Lemoy MJ, Westworth DR, Ardeshir A, Tarara RP. Reference intervals for preprandial and postprandial serum bile acid in adult rhesus macaques (Macaca mulatta). J Am Assoc Lab Anim Sci. 52(4):444-7, 2013. PMCID: PMC3725928, PMID 23849441

Yee J, Montiel NA, Ardeshir A, Lerche NW. Constitutive release of interferon (IFN)-γ and interleukin (IL)-2 from peripheral blood mononuclear cells of healthy rhesus macaques (Macaca mulatta) infected with simian T-lymphotropic virus type 1 (STLV1). Comp Med. 63(6): 508-514. 2013.PMID 24326227

Ardeshir A, Oslund KL, Ventimiglia F, Yee J, Lerche NW, and Hyde DM. Idiopathic microscopic colitis of rhesus macaques: Quantitative assessment of colonic mucosa. Anat Rec (Hoboken) 296: 1169-1179, 2013. PMID 23775860

Jones-Engel L, Feeroz M, Soliven K, Small C, Engel G, Pacheco A, Yee J, Wang X, Hasa K, Oh G, Levine K, Alam S, Craig K, Jackson D, Lee E, Barry P, Lerche N, Escalante A, Matsen F, Lineal M. Population dynamics of rhesus macaques and associated foamy virus in Bangladesh. EMI 2013. PMCID: PMC3675400

Van Rompay KK. The use of nonhuman primate models of HIV infection for the evaluation of antiviral strategies. AIDS Res Hum Retroviruses 28(1):16-35, 2012. PMID 21902451

Jensen K, Ranganathan UDK, Van Rompay KKA, Canfield DR, Khan I, Ravindran R, Luciw PA, Jacobs WR, Fennelly G, Larsen M, and Abel K. A recombinant attenuated Mycobacterium tuberculosis vaccine strain is safe in immunosuppressed SIV-infected infant macaques. Clin Vaccine Immunol 19:1170-81 2012. PMCID: PMC3416096, PMID 22695156

Montiel NA, Todd PA, Yee J, and Lerche NW. Effects of simian betaretrovirus serotype 1 (SRV1) infection on the differentiation of hematopoietic progenitor cells (CD34+) derived from bone marrow of rhesus macaques (Macaca mulatta). Comp Med 62: 61-68, 2012. PMCID: PMC3276394, PMID 22330653

Broadhurst MJ, Ardeshir A, Kanwar B, Mirpuri J, Gundra UM, Leung JM, Wiens KE, Vujkovic-Cvijin I, Kim CC, Yarovinsky F, Lerche NW, McCune JM, and Loke Pn. Therapeutic helminth infection of macaques with idiopathic chronic diarrhea alters the inflammatory signature and mucosal microbiota of the colon. PLoS Pathog 8: e1003000, 2012. PMCID: PMC3499566, PMID 23166490

Marthas ML, Van Rompay KK, Abbott Z, Earl P, Buonocore-Buzzelli L, Moss B, Rose NF, Rose JK, Kozlowski PA, Abel K. Partial efficacy of a VSV-SIV/MVA-SIV vaccine regimen against oral SIV challenge in infant macaques. Vaccine 29(17):3124-37, 2011. PMCID: PMC3078946, PMID 21377510

Kelly KR, Pypendop BH, Grayson JK, Stanley SD, Christe KL, Summers LM, and Lerche NW. Pharmacokinetics of oxymorphone in titi monkeys (Callicebus spp.) and rhesus macaques (Macaca mulatta). J Am Assoc Lab Anim Sci. 50: 212-20, 2011. PMCID: PMC3061422, PMID 21439215

Chen EC, Yagi S, Kelly KR, Mendoza SP, Tarara RP, Canfield DR, Maninger N, Rosenthal A, Spinner A, Bales KL, Schnurr DP, Lerche NW, and Chiu CY. Cross-species transmission of a novel adenovirus associated with a fulminant pneumonia outbreak in a new world monkey colony. PLoS Pathog 7: e1002155, 2011. PMCID: PMC3136464, PMID 21779173

White JA, Yang X, Todd PA, Lerche NW. Longitudinal patterns of viremia and oral shedding of rhesus rhadinovirus and retroperitoneal fibromatosis herpesviruses in age-structured captive breeding populations of rhesus macaques (Macaca mulatta). Comp Med 61(1):60-70, 2011. PMCID: PMC3060420, PMID 21819683

Luciw PA, Oslund KL, Yang X-w, Adamson L, Ravindran R, Canfield DR, Tarara R, Hirst L, Christensen M, Lerche NW, Offenstein H, Lewinsohn D, Ventimiglia F, Brignolo L, Wisner ER, and Hyde DM. Stereologic analysis of bacterial load and lung lesions in nonhuman primates (rhesus macaques) experimentally infected with Mycobacterium tuberculosis. Am J Physiol Lung Cell Mol Physiol 301: 731-738, 2011. PMCID: PMC3213984, PMID 21873450

Liao Q, Guo H, Tang M, Touzjian N, Lerche NW, Lu Y, and Yee JL. Simultaneous detection of antibodies to five simian viruses in nonhuman primates using recombinant viral protein based multiplex microbead immunoassays. J Virol Methods 178: 143-152, 2011. PMCID: PMC3213204, PMID 21945221

Van Rompay KK, Abel K, Earl P, Kozlowski PA, Easlick J, Moore J, Buonocore-Buzzelli L, Schmidt KA, Wilson RL, Simon I, Moss B, Rose N, Rose J and Marthas ML. Immunogenicity of viral vector, prime-boost SIV vaccine regimens in infant rhesus macaques: attenuated vesicular stomatitis virus (VSV) and modified vaccinia Ankara (MVA) recombinant SIV vaccines compared to live-attenuated SIV. Vaccine 28(6):1481-1492, 2010. PMCID: PMC2822069, PMID 19995539

von Gegerfelt A, Valentin A, Alicea C, Van Rompay KK, Marthas ML, Montefiori DC, Pavlakis GN, and Felber BK. Emergence of Simian immunodeficiency virus-specific cytotoxic CD4+ T Cells and increased humoral responses correlate with control of rebounding viremia in CD8-depleted macaques infected with rev-independent live-attenuated Simian immunodeficiency virus. J Immunol 185(6):3348-58, 2010. PMID 20702730

Winters MA, Van Rompay KK, Kashuba AD, Shulman NS, and Holodniy M. Maternal-fetal pharmacokinetics and dynamics of a single intrapartum dose of maraviroc in rhesus macaques. Antimicrob Agents Chemother 54(10):4059-4063, 2010. PMCID: PMC2944591, PMID 20696881

Van Rompay KK. Evaluation of antiretrovirals in animal models of HIV infection. Antiviral Res 85(1):159-175, 2010. PMID 19622373

Zao CL, Armstrong K, Tomanek L, Cooke A, Berger R, Estep JS, Marx PA, Trask JS, Smith DG, Yee JL, and Lerche NW. The complete genome and genetic characteristics of SRV-4 isolated from cynomolgus monkeys (Macaca fascicularis). Virology 405: 390-396, 2010. PMCID: PMC2941434, PMID 20615522

Kanthaswamy S, Kou A, Satkoski J, Penedo MC, Ward T, Ng J, Gill L, Lerche NW, Erickson B J, and Smith DG. Genetic characterization of specific pathogen-free rhesus macaque (Macaca mulatta) populations at the California National Primate Research Center (CNPRC). Am J Primatol 72: 587-599, 2010. PMCID: PMC2941796, PMID 20162538

Oates-O’Brien RS, Farver TB, Anderson-Vicino KC, McCowan B, and Lerche NW. Predictors of matrilineal overthrows in large captive breeding groups of rhesus macaques (Macaca mulatta). J Am Assoc Lab Anim Sci 49: 196-201, 2010. PMCID: PMC2846008, PMID 20353695

Lerche NW. Simian retroviruses: infection and disease – implications for immunotoxicology research in primates. J Immunotoxicol 7: 93-101, 2010. PMID 20433415

Olivier KJ, Jr, Price KD, Hutto DL, Lerche NW, Mansfield KG, Simmons JH, Taylor K, Myers L P, Ouyang Y, and Evans EW. Naturally occurring infections in non-human primates (NHP) and immunotoxicity implications: discussion sessions. J Immunotoxicol 7: 138-146, 2010. PMID 20441554

Farkas T, Cross RW, Hargitt E, 3rd, Lerche NW, Morrow AL, and Sestak K. Genetic diversity and histo-blood group antigen interactions of rhesus enteric caliciviruses. J Virol 84: 8617-8625, 2010. PMCID: PMC2919043, PMID 20554772

Zao CL, Armstrong K, Tomanek L, Cooke A, Berger R, Estep JS, Marx PA, Trask JS, Smith DG, Yee JL, and Lerche NW. The complete genome and genetic characteristics of SRV-4 isolated from cynomolgus monkeys (Macaca fascicularis). Virology 405: 390-396, 2010. PMCID: PMC2941434, PMID 20615522

Chen CI, Clark DC, Pesavento P, Lerche NW, Luciw PA, Reisen WK, and Brault AC. Comparative pathogenesis of epidemic and enzootic Chikungunya viruses in a pregnant rhesus macaque model. Am J Trop Med Hyg 83: 1249-1258, 2010. PMCID: PMC2990040, PMID 21118930

Durand-Gasselin L, Van Rompay KK, Vela JE, Henne IN, Lee WA, Rhodes GR, and Ray AS. Nucleotide analogue prodrug Tenofovir Disoproxil enhances lymphoid cell loading following oral administration in monkeys. Mol Pharm 6(4):1145-51, 2009. PMCID: PMC2777719, PMID 19545170

Fairman J, Moore J, Lemieux M, Van Rompay KK, Geng Y, Warner J, and Abel K. Enhanced in vivo immunogenicity of SIV vaccine candidates with cationic liposome-DNA complexes in a rhesus macaque pilot study. Hum Vaccin 5(3):141-50, 2009. PMCID: PMC2728146, PMID 18690014

Rosati M, Bergamaschi C, Valentin A, Kulkarni V, Jalah R, Alicea C, Patel V, von Gegerfelt AS, Montefiori DC, Venzon DJ, Khan AS, Draghia-Akli R, Van Rompay KK, Felber BK, and Pavlakis GN. DNA vaccination in rhesus macaques induces potent immune responses and decreases acute and chronic viremia after SIVmac251 challenge. Proc Natl Acad Sci U S A 106(37):15832-6, 2009. PMCID: PMC2734879, PMID 19717425