Go to UC Davis Search UC Davis  
  California National Primate Research Center Contact Us   |   Text Size   Enlarge text size   Reduce text size
 
 
 
Analytical and Resource Core  
The Analytical and Resource Core provides services and resources to the scientific research community in areas including hematology, clinical chemistry, genetics, immunology, endocrinology, flow cytometry, and pathogen detection. Scientists and staff associated with each of the seven Core Laboratories provide consultation in experimental design, sample collection, and data analysis, and offer assays that utilize species-specific reagents wherever possible. Core Laboratory scientists can also work with users to develop new assays to meet research needs. Training is available for all assays, and Core Laboratories equipment can be made available, typically on a recharge basis. Nonhuman primate resources developed at CNPRC are available to qualified individuals via the Resource Services component of the Core.
 
Clinical Laboratory
The Clinical Laboratory provides both clinical and research support in the areas of hematology, microbiology, chemistry, parasitology, serum preparation, urinalysis, and flow cytometry (through the Flow Cytometry Core Laboratory). Basic laboratory tests are provided in all the above areas of clinical laboratory medicine. Please contact for specific panels available for serum chemistries.  

For more information please contact:

Abigail Spinner
Clinical Laboratory Supervisor
Email:
(530) 752-5123

For rate information contact:
Abigail Spinner
aspinner@primate.ucdavis.edu

 
Endocrine Core Laboratory
The Endocrine Core Laboratory provides a service of hormone analysis as well as consultation relating to endocrine research for investigators. This core represents the combined services of two semi-independent laboratories, one emphasizing analysis of biological specimens for reproductive hormones and the other for analysis of stress hormones. In addition, this core develops and validates steroid, monoamine, and protein hormone assays for use in research conducted with nonhuman primates. Services include steroid and protein hormone immunoassays in a variety of species from urine, saliva, or blood samples as well as cell-based bioassays in serum samples. Routine immunoassays include monkey chorionic gonadotropin, cortisol, testosterone, estrogen, progesterone, prolactin, ACTH, thyroid hormones, and urinary metabolites of estrogen, progesterone and follicle stimulating hormone. Routine bioassays include bioactive androgens, estrogens, and luteinizing hormone. Other assays can be developed as needed by investigators. We can also offer assistance in experimental design and collection of samples, assessment of stress, and reproductive status.   For more information please contact:

Sally Mendoza, Ph.D.
(stress-related hormones)
Email:
(530) 752-1988
Rate Information

Bill L. Lasley, Ph.D.
(reproductive hormones)
Email:
(530) 752-8662
Rate Information
 
Flow Cytometry Core Laboratory
The Flow Cytometry Core Laboratory at the CNPRC provides access to, and services utilizing, state-of-the-art instruments. Currently the Laboratory has a FACS Calibur, which can be used for standard 4-color assays, and a FACS Aria, which has 3 lasers and can be used for multicolor flow cytometric analysis and for live cell sorting. Also available is a BD Fortessa 5 Laser Flow Cytometer, with a UV laser expanding the capability to do CaFlux assays and Hoechst staining. Investigators interested in using these instruments can choose to run their own assays or have their samples run by a trained technician on a recharge basis. Training in the use of the instruments is available from the Flow Cytometry Core Laboratory supervisor. Cell sorting will only be performed by the Core Supervisor and trained technicians supervised by her.

The Flow Cytometry Core will perform the staining, acquisition, and analysis of standard Clinical Lab panels, but PI's can request custom panels. Specific scientific questions relating to the development of new flow cytometric assays will be addressed by staff from the Infectious Disease Immunology Core Laboratory and Respiratory Disease Immunology Core Laboratory at the CNPRC, with whom the Flow Cytometry Core staff collaborate closely.
 

For more information please contact:

Abigail Spinner
Flow Cytometry Core Laboratory Supervisors
Email: aspinner@primate.ucdavis.edu
(530) 752-5123

For rate information contact:
Abigail Spinner
aspinner@primate.ucdavis.edu

 
Genetics Core Laboratory
The Genetics Core Laboratory provides research support in the following areas:
  • Genetic management including country of origin testing, parentage testing and other phylogenetic and population genetic analyses
  • Analysis of genealogical and demographic data including kinship assessment, pedigree validation, and the management of selective breeding and development of informative pedigrees
  • Bioinformatic analysis including whole genome and target region linkage and association analyses and candidate gene evaluation
  • Custom DNA extraction and purification, whole genome amplification, SNP, STR and mtDNA assays
  • Development of genotyping assays based on conventional fragment size analysis (including STRs and RFLPs), Sanger sequencing, CNV, SNP and real-time qPCR techniques
  For more information please contact:

Sree Kanthaswamy, Ph.D.
Genomics and Genetics Core Laboratory
Email:
(530) 752-1588; 2-0656
For rates contact Dr. Sree Kanthaswamy
 
Infectious Diseases Immunology Core Laboratory

The mission of the Infectious Diseases Immunology Core Laboratory is to provide interested researchers with assistance in the development of nonhuman primate infectious disease models and experiment planning. In addition, the goal of the Core Laboratory is to develop and provide the necessary tools for the analysis of nonhuman primate immune responses in these models. Currently, the following services are offered: blood and tissue processing (including mucosal tissues), PBMC isolation, antibody ELISAs against whole SIV and Hepatitis B, flow cytometric assays for phenotypic and functional characterization of multiple cell types (through the Flow Cytometry Core Laboratory), and RNA / DNA isolation (blood, saliva, urine, cell and tissue), cDNA preparation, real time PCR assays for gene expression profile study and absolute gene quantification, primer-probe design and optimization.

The Luminex multiplex microbead assay for the detection of antibodies and multiple immunomodulators in plasma or culture supernatants is available through two other cores (Pathogen Detection Laboratory and UCDMC Proteomics Core Laboratory); this includes use of multiplex panels from commercial sources as well as custom assay development.

Consultation in experimental design, technical training in the operation of instruments, trouble shooting and data analysis are available. Please inquire for additional assays or assay development. Assay development and standardization will be coordinated among this Core Laboratory, Respiratory Disease Immunology Core Laboratory, and Pathogen Detection Laboratory.

SIVmac239 and SIVmac251 virus stock is available through the Resource Services component of the Analytical and Resource Core.

Back to top

 

For more information please contact:

Koen VanRompay, D.V.M., Ph.D.
Email: kkvanrompay@ucdavis.edu
(530) 752-5281

 


Rate Information

 
Pathogen Detection Core Laboratory
The Pathogen Detection Core Laboratory, which has been in existence since 1986, offers antibody, antigen, virus, DNA, RNA and other diagnostic marker detection for a number of infectious agents including HIV, HVP2 (Herpes B surrogate marker), LCV, RRV, SFV, SIV, SRV, STLV, SV40, TB, RhCMV and other non human primate pathogens. All methods and reagents, including EIA, MMIA, WB, IFA, PCR, IHC, ISH and culture, are validated, standardized, and controlled by in-laboratory studies and proficiency testing using reference banks of characterized specimens. Quantitation is available by arrangement. Test results are reviewed by clinical laboratory personnel who are fully accredited by the American Society for Clinical Pathology and Laboratory Field Services of the State of California.

Custom testing, training, and consultation are available; please inquire. In addition, reagents and controls are available through Resource Services. PDCL is a resource for Specific Pathogen-Free (SPF) colonies, field or epidemiological studies, research protocols, and atypical case resolution.
  For more information please contact:

Joann Yee
Email: joyee@ucdavis.edu
(530) 752-8242
Rate Information

Visit our website at http://pdl.primate.ucdavis.edu
 
Respiratory Disease Immunology Core Laboratory

The objective of the Respiratory Disease Immunology Core Laboratory is to provide technical expertise and resources to investigators interested in the immunological evaluation of rhesus macaque models for lung disease. Studies are primarily carried out in conjunction with the Inhalation Exposure Core and the Computational Imaging Core, in order to provide a comprehensive evaluation of pathophysiological responses in the lung.

Services provided by the RDICL include assistance with experimental design, allergen characterization and preparation (through Resource Services), evaluation of allergen-specific IgE and IgG, and quantitation of histamine. The Core Laboratory also provides technical support for sample processing, including bronchoalveolar lavage, lung tissue, and blood. Flow cytometry and molecular analysis of lung and blood samples are coordinated with the Flow Cytometry Core Laboratory and the Infectious Disease Immunology Core Laboratory.

In addition to immunological evaluation, the Respiratory Disease Immunology Core Laboratory can provide investigators with primary cell isolates and in vitro culture of rhesus lung epithelial cells and fibroblasts. The Core does not conduct studies with human lung cultures; investigators interested in these services should contact Drs. Richart Harper and Nicholas Kenyon (UCDMC) for assistance. Please inquire for additional assays that are in development. In the future, immunohistochemistry of lung samples will be offered in conjunction with the Computational Imaging Core.

  For more information please contact:

Lisa A. Miller, Ph.D.
Email:
(530) 754-7546
Rate Information
 
Affiliated Laboratory: Clinical Proteomics Core Laboratory

In addition to the seven ARC laboratories, this Core has formal links to two Affiliated Laboratories located off-site. These Laboratories are directed by scientists associated with the CNPRC, and while they provide a broad range of services to the research community (including assays on human tissue), they also provide services to nonhuman primate researchers using species-specific reagents.

The Clinical Proteomics Core Laboratory in the UC Davis School of Medicine offers bead-based multiplex immunoassay test systems to enable the detection and measurement of multiple proteins simultaneously and quantitatively in a single test sample. Instrumentation consists of both the Luminex and Bio-Plex systems. Core services are organized into three components: (1) client-driven custom assay development (2) analyte detection by commercial kits (e.g., multiple cytokines and chemokines; biomarkers for cancer and other diseases), and (3) serodetection for infectious agents. The services offered through the Core Laboratory extend to all researchers and clinicians at UC Davis as well as other institutions. In addition, the Clinical Proteomics Core provides specialized expertise in the analysis and computational modeling of complex data generated on multiple analytes in preclinical and clinical samples.

  Contact Information:

Paul Luciw, Ph.D.,
Department of Pathology and Laboratory Medicine
UC Davis School of Medicine
Email:
 
Affiliated Laboratory: Host Microbe and Systems Biology Core

This Core is formally linked to the Analytical Core of the CNPRC and is located at the Genome and Biomedical Science Building.  The Host Microbe Systems Biology (HMSB) Core is directed by a Core Scientist at the CNPRC with expertise in nonhuman primate models of human diseases. The HMSB Core is designed to provide nonhuman primate researchers with easily accessible, customized, and cost-effective resources for integrating analyses of microbial community structure and host-microbe interactions in health and disease.  The Core provides state-of-the-art services for analyzing changes in the composition and diversity of the nonhuman primate microbiome through Illumina-based Next-Generation Sequencing (NGS), and genome-scale high throughput gene expression profiling using GeneChips.  The Core’s services also include consultation on experimental design and appropriate protocols for sample collection and processing.

 

  Contact Information:

Satya Dandekar, Ph.D.,
Department of Medical microbiology and Immunology
Infectious Diseases Unit, CNPRC
Email: sdandekar@ucdavis.edu

Matthew Rolston
Manager
Host-Microbe Systems Biology Core
Email: mrrolston@ucdavis.edu

Phone: (530) 754-7850
Resource Services

The following research resources of CNPRC are available to scientists on a recharge basis.

Allergen
Characterized protein extracts of house dust mite (Dermatophagoides pteronyssinus and Dermatophagoides farinae) are available for allergen sensitization projects.
Contact: Lisa Miller, Ph.D.


Biological Specimens
Tissues collected at necropsy are available from rhesus monkeys (Macaca mulatta), cynomolgus monkeys (Macaca fascicularis), and titi monkeys (Callicebus cupreus).
Contact:

Blood samples are available through our blood donor program.
Contact: Sarah Davis

or
Linda Hirst


Data
Data for colony animals are available from our computerized database. Data include birth records, weights, reproductive history, relocation history, etc.
Contact: John Capitanio, Ph.D.


DNA
DNA extracted from peripheral blood mononuclear cells is available on animals of all age-sex classes from known pedigrees.
Contact: Sree Kanthaswamy Ph.D.
skanthaswamy@ucdavis.edu

Reagents and Samples
Reagents, controls, and known/unknown samples are available from the Pathogen Detection Core Laboratory. Samples include pedigreed sera/plasma, fixed tissues and DNA from macaques and various other species. Validated reagents for many pathogens are available, including SIV, SRV1-5, SFV, STLV, RRV, RhCMV, Herpes B, SV40, and LCV. More information is available at: http://pdl.primate.ucdavis.edu/PDLreagents.html.
Contact:

Shipping
Shipping services are available by trained staff who can properly document, package and ship critical experimental materials, including nonhuman primate samples. Assistance is also provided for obtaining CITES permits, required for international shipment of any nonhuman primate samples.
Contact: Sarah Davis, , or Jenny Short,

Transformed B-Cell Lines
Cryopreserved Herpes papio - transformed B cell lines from over 300 rhesus monkeys in the CNPRC colony are available. Transformation of macaque B cells to establish a new cell line is available on request.
Contact: Michael B. McChesney, M.D.,

Virus Stock: Rhesus Cytomegalovirus:
A unique primary isolate, developed at CNPRC, is available.
Contact: Peter A. Barry, Ph.D.,

Virus Stock: Simian Immunodeficiency Virus
Aliquots of SIVmac251 and SIVmac239 virus stocks were prepared by propagation in peripheral blood mononuclear cells from rhesus macaques and contain approximately 100,000 50% tissue culture infectious doses per ml. As measured by the commercial SIV branched chain assay, SIVmac251 contains 2 x 109 copies of SIV RNA per ml and SIVmac239 contains 109 copies of SIV RNA per ml. These virus stocks are infectious for rhesus macaques by intravenous, intravaginal and oral routes of inoculation.
Contact: Koen VanRompay, Ph.D.,